An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo.
Aubrey BJ, Kelly GL, Kueh AJ, Brennan MS, O'Connor L, Milla L, Wilcox S, Tai L, Strasser A, Herold MJ
Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70182 | FUCas9Cherry | Expresses Cas9 with fluorescent Cherry reporter |
| 70183 | FgH1tUTG | Inducible expression of guide RNA with fluorescent GFP reporter |
| 85530 | FgH1tUTG_huMcl-1.1 | Inducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporter |
| 85531 | FgH1tUTG_huMcl-1.2 | Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter |
| 85532 | FgH1tUTG_huBim_Ex3 | Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter |
| 85533 | FgH1tUTG_muBim_Ex3 | Inducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporter |
| 85534 | FgH1tUTG_mup53_Ex4 | Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter |
| 85535 | FgH1tUTG_mup53_Ex5 | Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter |