Transcription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants.
Meyer AJ, Garry DJ, Hall B, Byrom MM, McDonald HG, Yang X, Yin YW, Ellington AD
Nucleic Acids Res. 2015 Sep 3;43(15):7480-8. doi: 10.1093/nar/gkv734. Epub 2015 Jul 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70172 | pQE-WT-RGVG-M6 | Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA |