B12 cofactors directly stabilize an mRNA regulatory switch.
Johnson JE Jr, Reyes FE, Polaski JT, Batey RT
Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70127 | pENV8(WT)-GFPuv | Cobalamin riboswitch reporter vector for use in E. coli |
| 70128 | pENV8(GAAA)-GFPuv | Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop |