Engineering a Circular Riboregulator in Escherichia coli.
Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69926 | circRAJ31 | Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. |
| 69928 | cisRAJ31-GFP-LAA | Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31. |
| 69930 | circRAJ31-Toehold_mutant | Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31. |
| 69931 | pT7_circRAJ31 | Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter. |
| 69932 | pT7_circRAJ31_C873U | Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter. |
| 69933 | cisRAJ31-cat | Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31. |
| 69934 | taRAJ31min | Expresses taRAJ31min, a linear riboregulator. |