Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.
Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T
Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69534 | CAG-DDdCas9VP192-T2A-EGFP-ires-puro | DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim. |
| 69535 | pCXLE-dCas9VP192-T2A-EGFP-shP53 | Episomal plasmid encoding dCas9VP192 and p53 shRNA |
| 69536 | pCXLE-dCas9VP192-T2A-EGFP | Episomal plasmid encoding dCas9VP192 |
| 69537 | GG-EBNA-OCT4 | Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter |
| 69538 | GG-dest | Destination plasmid for gRNA concatenation Golden Gate cloning |