Autophosphorylation of polo-like kinase 4 and its role in centriole duplication.
Sillibourne JE, Tack F, Vloemans N, Boeckx A, Thambirajah S, Bonnet P, Ramaekers FC, Bornens M, Grand-Perret T
Mol Biol Cell. 2010 Feb 15;21(4):547-61. doi: 10.1091/mbc.E09-06-0505. Epub 2009 Dec 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69837 | pEGFP-C3-PLK4-3xFLAG | This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag |
| 69838 | pEGFP-C3-PLK4 K41M-3xFLAG | This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag |
| 69839 | pEGFP-C3-PLK4-S305A-3xFLAG | This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag |
| 69840 | pEGFP-C3-PLK4-S305E-3xFLAG | This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag |
| 69841 | pEGFP-C3-PLK4-S285A/T289A-3xFLAG | This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag |
| 69842 | pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG | This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag |