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Tandem SUMO fusion vectors for improving soluble protein expression and purification.

Guerrero F, Ciragan A, Iwai H
Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 10.1016/j.pep.2015.08.019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64692pLJSRSF3His-GST-Smt3-NpuDnaEN102-CBD
64693pLJSRSF7His-MBP-Smt3-NpuDnaEN102-CBD
64694pCARSF85H6-TF-Smt3-GB1-NpuDnaEN102-CBD
64695pCARSF63H6-TRX-Smt3-GB1-NpuDnaEN102-CBD
64696pHYRSF53His-Smt3-NpuDnaEN102-CBD
64697pFGET19_Ulp1H6-Ulp1 from pHYRS52 into pET-28b(+)

Antibodies from Article