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Simultaneous characterization of cellular RNA structure and function with in-cell SHAPE-Seq.

Watters KE, Abbott TR, Lucks JB
Nucleic Acids Res. 2015 Sep 8. pii: gkv879. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69455pJBL-RNAINS3RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69456pJBL-RNAINS4RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69457pJBL-RNAOUTA3RNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69458pJBL-RNAOUTA4RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69461pJBL-RNAINS4-A25CRNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69462pJBL-RNAINS4-C24ARNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69463pJBL-RNAINS4-C24AA25CRNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
69464pJBL-anti-controlAntisense control vector for functional testing (ColE1 ori, AmpR, strong promoter/terminator)

Antibodies from Article