Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G
Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68495 | AAV_NLS-dSaCas9-NLS-VPR | AAV vector containing nuclease null SaCas9 fused to VPR |
| 68496 | AAV_NLS-SaCas9-NLS-VPR | AAV vector containing SaCas9 fused to VPR |
| 68497 | hCas9-VPR | nuclease competent SP-Cas9 fused to VPR |
| 68498 | M-ST1-VPR | nuclease competent ST1-Cas9 fused to VPR |
| 79368 | gRNA_tdtomato_reporter_1_ST1_2x_binding | Assays activity of transcriptional activators fused to ST1 Cas9 |
| 79369 | gRNA_tdtomato_reporter_1_SA-2x | Assays activity of transcriptional activators fused to SA Cas9 |
| 79370 | gRNA_tdtomato_reporter1_SP-2x | Assays activity of transcriptional activators fused to SP Cas9 |