Skip to main content
Addgene

Charles Gersbach Lab: TMG reporters

Gersbach
Unpublished

Plasmids from Article

ID Plasmid Purpose
69545tetO-GFPnls-mPGK-rtTA-IRES-PuroTet-On system expressing GFP tagged with 2x nuclear localization signals.
69546tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-PuroTet-On system expressing MyoD-2A-GFP-2xNLS.
69547Myog'-2A-mCherryNLS-PGK-PurodtkDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
69548Myog'-2A-mCherryNLS-PGK-PuroDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
69549pZDonor-U6-myogenin stop codon gRNAExpresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
69550FU-H2B-GFP-IRES-PuroLentiviral, selectable fluorescent GFP tagging of nuclei.

Antibodies from Article