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Identification of AMPK Phosphorylation Sites Reveals a Network of Proteins Involved in Cell Invasion and Facilitates Large-Scale Substrate Prediction.

Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE, Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
Cell Metab. 2015 Nov 3;22(5):907-21. doi: 10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69504pECE HA AMPKa1 WTExpresses AMPK alpha1 in mammalian cells
69811pECE M2-SNX17 WTExpresses M2-SNX17 in mammalian cells
69812pECE M2-SNX17 S437AExpresses M2-SNX17 in mammalian cells
69813pECE M2-SH3PXD2A WTExpresses M2-SH3PXD2A in mammalian cells
69814pECE M2-SH3PXD2A S974AExpresses M2-SH3PXD2A in mammalian cells
69815pECE M2-SH3PXD2A 6 serines mutated to alaninesExpresses M2-SH3PXD2A in mammalian cells
69816pECE M2-SH3PXD2A 8 serines mutated to alaninesExpresses M2-SH3PXD2A in mammalian cells
69817pcDNA 3.2/V5-DEST NET1A V5 WTExpresses NET1A-V5 in mammalian cells
69818pcDNA 3.2/V5-DEST NET1A S46AExpresses NET1A-V5 in mammalian cells
69819pcDNA 3.2/V5-DEST CDC42EP1 WTExpresses CDC42EP1-V5 in mammalian cells
69820pcDNA 3.2/V5-DEST CDC42EP1 S192AExpresses CDC42EP1-V5 in mammalian cells
69821pcDNA 3.2/V5-DEST SNAP29-V5Expresses SNAP29-V5 in mammalian cells
69822pcDNA 3.2/V5-DEST MTFR1L-V5Expresses MTFR1L-V5 in mammalian cells
69823pcDNA 3.2/V5-DEST RBM14-V5Expresses RBM14-V5 in mammalian cells
69824FUW TetO HA-AMPKa1 WTExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner
69825FUW TetO HA-AMPKa1 ASExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner
69826FUW TetO HA-AMPKa2 WTExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner
69827FUW TetO HA-AMPKa2 ASExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner
69828FUW TetO NET1A-V5 WTExpresses NET1A-V5 in mammalian cells in a doxycycline inducible manner
69829FUW TetO NET1A-V5 S46AExpresses NET1A-V5 in mammalian cells in a doxycycline inducible manner
69831pECE HA AMPKa1 ASExpresses Expresses AMPK alpha 1 in mammalian cells

Antibodies from Article