Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation.
Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC
ACS Synth Biol. 2014 Jun 20;3(6):398-409. doi: 10.1021/sb400140t. Epub 2014 Jan 2.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
69494 | rEc.13 | Recoded strain from MG1655 with prfA |
69495 | rEc.13.delA | Recoded strain from MG1655, prfA deleted |