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A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.

Lowder LG, Paul JW, Baltes NJ, Voytas DF, Zhang Y, Zhang D, Tang X, Zheng X, Hsieh TF, Qi Y
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69273pYPQ131AGolden Gate entry vector; 1st gRNA under AtU6 promoter
69274pYPQ132AGolden Gate entry vector; 2nd gRNA under AtU6 promoter
69275pYPQ133AGolden Gate entry vector; 3rd gRNA under AtU6 promoter
69276pYPQ134AGolden Gate entry vector; 4th gRNA under AtU6 promoter
69277pYPQ135AGolden Gate entry vector; 5th gRNA under AtU6 promoter
69278pYPQ136AGolden Gate entry vector; 6th gRNA under AtU6 promoter
69279pYPQ137AGolden Gate entry vector;7th gRNA under AtU6 promoter
69280pYPQ138A Golden Gate entry vector; 8th gRNA under AtU6 promoter
69281pYPQ131BGolden Gate entry vector; 1st gRNA under AtU3 promoter
69282pYPQ132BGolden Gate entry vector; 2nd gRNA under AtU3 promoter
69283pYPQ133B Golden Gate entry vector; 3rd gRNA under AtU3 promoter
69284pYPQ131CGolden Gate entry vector; 1st gRNA under OsU6 promoter
69285pYPQ132CGolden Gate entry vector; 1st gRNA under OsU6 promoter
69286pYPQ133CGolden Gate entry vector; 1st gRNA under OsU6 promoter
69287pYPQ131DGolden Gate entry vector; 1st gRNA under OsU3 promoter
69288pYPQ132DGolden Gate entry vector; 1st gRNA under OsU3 promoter
69289pYPQ133DGolden Gate entry vector; 1st gRNA under OsU3 promoter
69290pYPQ141AGateway entry vector; single gRNA under AtU6 promoter
69291pYPQ141BGateway entry vector; single gRNA under AtU3 promoter
69292pYPQ141CGateway entry vector; single gRNA under OsU6 promoter
69293pYPQ141DGateway entry vector; single gRNA under OsU3 promoter
69294pYPQ142Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs
69295pYPQ143Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs
69296pYPQ144Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs
69297pYPQ145 Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs
69298pYPQ146Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs
69299pYPQ147Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs
69300pYPQ148Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs
69301pYPQ150Gateway entry vector with pcoCas9 (Plant codon optimized)
69302pYPQ151Gateway entry vector with pcoCas9D10A
69303pYPQ152Gateway entry vector with pco-dCas9-VP64
69304pYPQ153Gateway entry vector with pco-dCas9-3X(SRDX)
69305pYPQ154Gateway entry vector with AteCas9 (Arabidopsis codon optimized)
69306pYPQ155Gateway entry vector with AteCas9D10A
69307pYPQ158Gateway entry vector with hSpCas9 (Human codon optimized)
69308pYPQ159Gateway entry vector with hSpCas9D10A
69309pYPQ167Gateway entry vector with Cas9p

Antibodies from Article