Assessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice.
Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato M, Ohtsuka M
PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62680 | pARA (pKD-G1) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62681 | pARB (pKD-G2) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62682 | pARC (pKD-G3) | Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer |
| 62683 | pARD (pKD-G4) | Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer |
| 62684 | pATG (pKD-G5) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62685 | pATH (pKD-G6) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62686 | pATI (pKD-G7) | Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer |
| 62687 | pATJ (pKD-G8) | Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer |
| 62688 | pATK (pKD-G9) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62689 | pATL (pKD-G10) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62690 | pATM (pKD-G11) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62691 | pATN (pKD-G12) | Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer |
| 62707 | pAQI (pT-1) | Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer |
| 62708 | pART (pT-2) | Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer |
| 62709 | pAYP (pDre) | Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer |
| 62710 | pAWV | Donor vector for amiR-eGFP knock-in via landing pad |
| 62713 | pAWK | Donor vector for amiR-eGFP knock-in via landing pad |