Dynamic single cell measurements of kinase activity by synthetic kinase activity relocation sensors.
Durandau E, Aymoz D, Pelet S
BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68274 | pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry | SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry |
| 68275 | pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry | SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS |
| 68276 | pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry | SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS |
| 68277 | pED55 pRS306 pRPS2-SteND-NLS-mCherry | SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation |
| 68278 | pED73 pRS304 pRPS2-SteDS-NLS-mCitrine | SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine |
| 68279 | pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry | SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry |
| 68280 | pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry | SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry |