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Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries.

Vidigal JA, Ventura A
Nat Commun. 2015 Aug 17;6:8083. doi: 10.1038/ncomms9083. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69312pDonor_hU6sgRNA scaffold and human U6 promoter
69350pDonor_mU6sgRNA scaffold and mouse U6 promoter
69351pDonor_sU6sgRNA scaffold and synthetic U6 promoter

Antibodies from Article