Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor.
de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
ACS Synth Biol. 2015 Aug 19.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69030 | pES001 (pQacA-deGFP) | Reporter Plasmid - deGFP driven by a qacR responsive promoter |
| 69031 | pES002 (pTet-qacR wt) | wild-type qacR downstream of a Tet promoter |
| 69032 | pES003 (pTet-qacR 2) | qacR 2 downstream of a Tet promoter |
| 69033 | pES004 (pTet-qacR 5) | qacR 5 downstream of a Tet promoter |
| 69034 | pES005 (pTet-qacR wt/pQacA-deGFP) | reporter with wild-type qacR on single plasmid |
| 69035 | pES006 (pTet-qacR 2/pQacA-deGFP) | reporter with qacR 2 on single plasmid |
| 69036 | pES007 (pTet-qacR 5/pQacA-deGFP) | reporter with qacR 5 on single plasmid |