Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei.
Alsford S, Horn D
Mol Biochem Parasitol. 2008 Sep;161(1):76-9. doi: 10.1016/j.molbiopara.2008.05.006. Epub 2008 Jun 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69242 | pRPa-c6MYCn | Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei. |
| 69243 | pRP-c6MYCn | Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei. |
| 69244 | pRPa-iSL(MCS1+2) | Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei. |
| 69246 | pRPa-iSL(MCS3+4) | Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei. |