Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum.
Gilles AF, Schinko JB, Averof M
Development. 2015 Jul 9. pii: dev.125054.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65955 | p(U6a-BsaI-gRNA) | Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence |
| 65956 | p(U6b-BsaI-gRNA) | Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence |
| 65959 | p(bhsp68-Cas9) | Tribolium basal heat shock promoter driving Cas9 |