Red fluorescent proteins for gene expression and protein localization studies in Streptococcus pneumoniae and efficient transformation with Gibson assembled DNA.
Beilharz K, van Raaphorst R, Kjos M, Veening JW
Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69102 | pKB01_mOrange2 | Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over. |
| 69103 | pKB01_tagRFP | Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over. |
| 69104 | pKB01_mCherry | Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over. |
| 69105 | pKB01-PssbB-mKate2 | Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over. |