A highly sensitive selection method for directed evolution of homing endonucleases.
Chen Z, Zhao H
Nucleic Acids Res. 2005 Oct 6;33(18):e154.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
69056 | p11-LacY-wtx1 | The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies. |