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A highly sensitive selection method for directed evolution of homing endonucleases.

Chen Z, Zhao H
Nucleic Acids Res. 2005 Oct 6;33(18):e154. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69056p11-LacY-wtx1The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Antibodies from Article