CHD5 is required for neurogenesis and has a dual role in facilitating gene expression and polycomb gene repression.
Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, Holmberg J, Bracken AP
Dev Cell. 2013 Aug 12;26(3):223-36. doi: 10.1016/j.devcel.2013.07.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68869 | pENTR3c GW CHD5 | GW entry vector for CHD5 |
| 68870 | pCMV-FLAG-CHD5-WT-331-672aa | Mammalian expression of CHD5 histone binding domain (331-672aa) |
| 68871 | pCMV-FLAG-CHD5-Y619E-FRAG | Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation |
| 68873 | pGEX-GST-CHD5-WT-FRAG | IPTG inducible bacterial expression of CHD5 fragment |
| 68874 | pGEX-GST-CHD5-L518A/Y619E-FRAG | IPTG inducible bacterial expression of CHD5 fragment |
| 68875 | pGEX-GST-CHD5(Chromos)-FRAG | IPTG inducible bacterial expression of CHD5 fragment |
| 68876 | pAAV-MCS-shCHD5 #1 | CHD5 shRNA expressed from Adeno-associated viral (AAV) vector |
| 68877 | pAAV-MCS-shCHD5 #2 | CHD5 shRNA expressed from Adeno-associated viral (AAV) vector |
| 68878 | pCAG-Flag-Avi-ires-CHD5 | Transient mammalian expression of CHD5 for BirA tagging |
| 68879 | pCR8-CHD5-FRAGMENT | GW entry vector for CHD5 |