Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, Weiss R
Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59560 | pTK095 | SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2 |
| 59561 | pTK101 | SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4 |
| 59562 | pTK105 | SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST |
| 59563 | pTK194 | TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI |
| 59564 | pTK295 | SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP |
| 59565 | pTK296 | SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PEST |
| 59566 | pTK297 | SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4 |
| 59567 | pTK298 | SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 |
| 59568 | pTK299 | SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 |
| 59569 | pTK312 | TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI |
| 59570 | pTK313 | TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI |
| 59571 | pTK317 | TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI |
| 59572 | pTK331 | TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI |
| 59573 | pTK332 | SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5 |
| 59574 | pTK333 | SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5 |