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Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota.

Mimee M, Tucker AC, Voigt CA, Lu TK
Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
68543pMM553NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR
68880pMM555NanoLuc expressed constiutively from PcfxA, pNBU2 backbone, AmpR
68881pMM575NanoLuc expressed constiutively from PcfiA, pNBU2 backbone, AmpR
68882pMM579NanoLuc expressed constiutively from P1, pNBU2 backbone, AmpR
68883pMM580NanoLuc expressed constiutively from PcepA, pNBU2 backbone, AmpR
68884pMM585IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR
68885pMM596IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR
68886pMM597IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR
68887pMM656Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR
68888pMM659Chondroitin sulfate-inducible NanoLuc expression, pNBU2 backbone, AmpR
68889pMM660Arabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpR
68890pMM668pExchange(tdk) - B. thetaiotaomicron suicide vector expressing thymidine kinase
68891pMM704IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR
68892pMM705IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR
68893pMM710IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR
68895pMM725IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR
68896pMM731IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR
68897pMM732IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR
68898pMM733IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR
68899pMM750IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR
68900pMM763IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR
68901pMM764IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR
68902pAT751NanoLuc expressed constiutively from PAM1, pNBU2 backbone, AmpR
68903pAT752NanoLuc expressed constiutively from PAM2, pNBU2 backbone, AmpR
68904pAT753NanoLuc expressed constiutively from PAM3, pNBU2 backbone, AmpR
68905pAT754NanoLuc expressed constiutively from PAM4, pNBU2 backbone, AmpR
68906pAT587NanoLuc expressed constiutively from PBT1311 and GH022 RBS, pNBU2 backbone, AmpR
68907pAT588NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR
68908pAT590NanoLuc expressed constiutively from PBT1311 and GH049 RBS, pNBU2 backbone, AmpR
68909pAT593NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR
68910pAT695NanoLuc expressed constiutively from PBT1311 and RC500 RBS, pNBU2 backbone, AmpR
68911pAT772NanoLuc expressed constiutively from PAM1 and GH078 RBS, pNBU2 backbone, AmpR
68913pAT774NanoLuc expressed constiutively from PAM1 and GH023 RBS, pNBU2 backbone, AmpR
68914pAT775NanoLuc expressed constiutively from PAM1 and rpiL* RBS, pNBU2 backbone, AmpR
68915pAT776NanoLuc expressed constiutively from PAM1 and RC500 RBS, pNBU2 backbone, AmpR
68916pAT779NanoLuc expressed constiutively from PAM2 and GH078 RBS, pNBU2 backbone, AmpR
68917pAT780NanoLuc expressed constiutively from PAM2 and GH022 RBS, pNBU2 backbone, AmpR
68918pAT781NanoLuc expressed constiutively from PAM2 and GH023 RBS, pNBU2 backbone, AmpR
68919pAT782NanoLuc expressed constiutively from PAM2 and rpiL* RBS, pNBU2 backbone, AmpR
68920pAT783NanoLuc expressed constiutively from PAM2 and RC500 RBS, pNBU2 backbone, AmpR
68921pAT786NanoLuc expressed constiutively from PAM3 and GH078 RBS, pNBU2 backbone, AmpR
68922pAT787NanoLuc expressed constiutively from PAM3 and GH022 RBS, pNBU2 backbone, AmpR
68923pAT788NanoLuc expressed constiutively from PAM3 and GH023 RBS, pNBU2 backbone, AmpR
68924pAT789NanoLuc expressed constiutively from PAM3 and rpiL* RBS, pNBU2 backbone, AmpR
68925pAT790NanoLuc expressed constiutively from PAM3 and RC500 RBS, pNBU2 backbone, AmpR
68926pAT793NanoLuc expressed constiutively from PAM4 and GH078 RBS, pNBU2 backbone, AmpR
68927pAT794NanoLuc expressed constiutively from PAM4 and GH022 RBS, pNBU2 backbone, AmpR
68928pAT795NanoLuc expressed constiutively from PAM4 and GH023 RBS, pNBU2 backbone, AmpR
68929pAT796NanoLuc expressed constiutively from PAM4 and rpiL* RBS, pNBU2 backbone, AmpR
68930pAT797NanoLuc expressed constiutively from PAM4 and RC500 RBS, pNBU2 backbone, AmpR
68931pAT890Int7 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR
68932pAT891Int8 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR
68933pAT892Int9 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR
68934pAT895Int12 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpR
68935pAT847Memory arrary integration vector for insertion between BT2113 and BT2114 in the B. thetaiotaomicron chromosome, AmpR
68936pAT937Rhamnose-inducible Int12 expression vector with rpiL*C51 RBS, pNBU2 backbone, AmpR
68941pAT773NanoLuc expressed constiutively from PAM1 and GH022 RBS, pNBU2 backbone, AmpR

Antibodies from Article