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A new expression vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson I+J phagemid libraries.

Ossysek K, Uchanski T, Kulesza M, Bzowska M, Klaus T, Wos K, Madej M, Bereta J
Immunol Lett. 2015 Jul 25. pii: S0165-2478(15)30011-0. doi: 10.1016/j.imlet.2015.07.005. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67843pET-scFv-TpET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

Antibodies from Article