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Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene.

Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC
Cell. 2007 Jun 15. 129(6):1065-1079. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
15263pBabe-GFP-IKBalpha-wt
15264pBabe-GFP-IKBalpha-mut (super repressor)
15265pBabe-Neo-Flag-IKBKE
15266pBabe-Neo-Myr-Flag-AKT1
15267pBabe-Neo-Myr-Flag-IKBKE
15268pBabe-Puro-MEK-DD
15269pBabe-Puro-BRAF-V600ERetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
15270pBabe-Puro-cRAF-W22
15290pBabe-Puro-IKBalpha-wt
15291pBabe-Puro-IKBalpha-mut (super repressor)
15292pBabe-Puro-Flag-IKBKE
15293pBabe-Puro-Myr-Flag-IKBKE
15294pBabe-Puro-Myr-Flag-AKT1
15301pENTRY-GFP
15302pENTRY-YFP

Antibodies from Article