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Dynamic visualization of transcription and RNA subcellular localization in zebrafish.

Campbell PD, Chao JA, Singer RH, Marlow FL
Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. Epub 2015 Mar 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
86242pME-mCherry-24xMBSMiddle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter
86243pME-NLS-tdMCP-eGFPMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter
86244pME-NLStdMCP- tagRFPMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFP
86245pME-NLS-tdMCPMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP
86246pME-24xMBSMiddle entry vector for generating MBS tagged RNAs
86248pTol-Promoter:NLS-tdMCP-eGFPFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Antibodies from Article