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CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.

Mans R, van Rossum HM, Wijsman M, Backx A, Kuijpers NG, van den Broek M, Daran-Lapujade P, Pronk JT, van Maris AJ, Daran JM
FEMS Yeast Res. 2015 Mar;15(2). pii: fov004. doi: 10.1093/femsyr/fov004. Epub 2015 Mar 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107916pMEL10URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107917pMEL11 amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107918pMEL12hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107919pMEL13 kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107920pMEL14 LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107921pMEL15 nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107922pMEL16 HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107923pMEL17 TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107924pROS10 URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107925pROS11 amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107926pROS12 hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107927pROS13 kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107928pROS14 LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107929pROS15 nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107930pROS16 HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer
107931pROS17 TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Antibodies from Article