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Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters.

MacKay JL, Kumar S
Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84605pSLIK CA Rac1Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus
84642pSLIK DN Rac1Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus
84643pCuo CA Rac1constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
84644pCuo DN Rac1dominant negative Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
84646pSLIK DN RhoALentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus
112894pSLIK CA RhoApromoter-inducible expression of constitutively-active RhoA

Antibodies from Article