Site-specific bacterial chromosome engineering: PhiC31 integrase mediated cassette exchange (IMCE).
Heil JR, Cheng J, Charles TC
J Vis Exp. 2012 Mar 16;(61). pii: 3698. doi: 10.3791/3698.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
68376 | pJH110 | Clone cassette to replace RFP for integration into C31 landing pad strain |