A modular open platform for systematic functional studies under physiological conditions.

Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S
Nucleic Acids Res. 2015 May 24. pii: gkv550. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
65626	pEX-A-U6-gRNA	Empty vector for the expression U6 driven gRNA
65625	pCAG-tagRFP-NLS-HA-Bxb1	Vector for cotranslational expression of tagRFP-Bxb1
65624	Bxb1-surrogate-reporter	Fluorescent reporter to monitor the activty of Bxb1
65561	attB-GFP-Tet1d834-1053-Poly(A)	Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus
65560	attB-GFP-Tet1d834-1363-Poly(A)	Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus
65559	attB-GFP-Tet1-Poly(A)	Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus
65556	attB-mCh-Dnmt3b1-Poly(A)-PuroR	Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection
65554	attB-mCh-Dnmt3b6-Poly(A)-NeoR	Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection
65553	attB-mCh-Dnmt3b1-Poly(A)-NeoR	Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection
65552	attB-GFP-Dnmt3b6-Poly(A)-NeoR	Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection