Jannik Elverløv-Jakobsen Lab: Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68347 | AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR | AAV plasmid expressing the FlpO recombinase. with empty gRNA |
| 68348 | px330_PTEN exon 5 gRNA | px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter. |
| 68349 | px330_P53 exon 8 gRNA | px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter. |
| 68350 | px330_SMAD exon 2 gRNA | px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter. |
| 68351 | px330_P53 exon 7 gRNA | px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter. |
| 68352 | px330_SMAD exon 9 gRNA | px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter. |
| 68356 | 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO | -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter |
| 68357 | 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO | The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter. |
| 68405 | 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO | The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter |