Computational redesign of the lipid-facing surface of the outer membrane protein OmpA.
Stapleton JA, Whitehead TA, Nanda V
Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62639 | pJexpress414_OmpA171_R4cons | Conservative version of OmpA Redesign 4 |
| 62646 | pJexpress414_OmpA171_WT | Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop |
| 62647 | pJexpress414_OmpA171_H1346 | OmpA redesign hybrid with wild-type strands 1346 |
| 62648 | pJexpress414_OmpA171_H1256 | OmpA redesign hybrid with wild-type strands 1256 |
| 62649 | pJexpress414_OmpA171_H2345 | OmpA redesign hybrid with wild-type strands 2345 |
| 62650 | pJexpress414_OmpA171_H3567 | OmpA redesign hybrid with wild-type strands 3567 |
| 62651 | pJexpress414_OmpA171_H2368 | OmpA redesign hybrid with wild-type strands 2368 |
| 62652 | pJexpress414_OmpA171_H1678 | OmpA redesign hybrid with wild-type strands 1678 |
| 62653 | pJexpress414_OmpA171_WTnoGirdles | Wild-type OmpA with aromatic girdle residues replaced by leucine |
| 64290 | pJexpress414_OmpA171_H1237 | OmpA redesign hybrid with wild-type strands 1237 |
| 64291 | pJexpress414_OmpA171_H2578 | OmpA redesign hybrid with wild-type strands 2578 |
| 64292 | pJexpress414_OmpA171_H3478 | OmpA redesign hybrid with wild-type strands 3478 |
| 64293 | pJexpress414_OmpA171_H1248 | OmpA redesign hybrid with wild-type strands 1248 |
| 64294 | pJexpress414_OmpA171_H2467 | OmpA redesign hybrid with wild-type strands 2467 |
| 64295 | pJexpress414_OmpA171_H1457 | OmpA redesign hybrid with wild-type strands 1457 |
| 64296 | pJexpress414_OmpA171_H4568 | OmpA redesign hybrid with wild-type strands 4568 |
| 64297 | pJexpress414_OmpA171_H1358 | OmpA redesign hybrid with wild-type strands 1358 |
| 64298 | pJexpress414_OmpA171_R4cons_G125L | Conservative version of OmpA Redesign 4 with G125L mutation |
| 64299 | pJexpress414_OmpA171_R4cons_G41A | Conservative version of OmpA Redesign 4 with G41A mutation |
| 64300 | pJexpress414_OmpA171_R4cons_G99I | Conservative version of OmpA Redesign 4 with G99I mutation |
| 64301 | pJexpress414_OmpA171_R4cons_P121F | Conservative version of OmpA Redesign 4 with P121F mutation |
| 64302 | pJexpress414_OmpA171_R4cons_Y129H | Conservative version of OmpA Redesign 4 with Y129H mutation |
| 64310 | pJexpress414_OmpA171_H-3 | OmpA redesign hybrid with wild-type strands 1245678 |
| 64311 | pJexpress414_OmpA171_H-46 | OmpA redesign hybrid with wild-type strands 123578 |
| 64312 | pJexpress414_OmpA171_H346 | OmpA redesign hybrid with wild-type strands 346 |
| 64313 | pJexpress414_OmpA171_H-78 | OmpA redesign hybrid with wild-type strands 123456 |