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Synthetic biosensors for precise gene control and real-time monitoring of metabolites.

Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
Nucleic Acids Res. 2015 Jul 7. pii: gkv616. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62557pJKR-H-cdaRGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.
62558pJKR-L-cdaRGlucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.
62559pJKR-H-mphRMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.
62560pJKR-L-mphRMacrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.
62561pJKR-H-tetRTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.
62562pJKR-L-tetRTetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.
62563pJKR-H-araCArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.
62565pJKR-H-ttgRFlavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.
62566pJKR-L-ttgRFlavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.
62567pJKR-H-acuRAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.
62568pJKR-L-acuRAcrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.
62569pJKR-O-acuRAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.
62570pJKR-O-mphRMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Antibodies from Article