Arabidopsis PIAL1 and 2 promote SUMO chain formation as E4-type SUMO ligases and are involved in stress responses and sulfur metabolism.

Tomanov K, Zeschmann A, Hermkes R, Eifler K, Ziba I, Grieco M, Novatchkova M, Hofmann K, Hesse H, Bachmair A
Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300. Epub 2014 Nov 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
67908	pMAL-PIAL2M-sim1	Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.
67909	pMAL-PIAL2M-wt	Inducible expression of PIAL2 in E.coli