Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes.

Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
58693	N-Terminal Split Cas9 with GyrA intein	Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
58694	C-Terminal Split Cas9 with GyrA intein	Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
58695	N-Terminal Split Cas9 D10A Nickase with GyrA intein	Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production