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RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme.

Holzmann J, Frank P, Loffler E, Bennett KL, Gerner C, Rossmanith W
Cell. 2008 Oct 31;135(3):462-74. doi: 10.1016/j.cell.2008.09.013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67862pET28-MRPP1bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)
67863pET28-HADH2bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)
67864pET28-MRPP3-myc-Hisbacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Antibodies from Article