Gene circuit performance characterization and resource usage in a cell-free "breadboard".
Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. Epub 2014 Apr 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67734 | Pr-deGFP-MGapt | OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion |
| 67735 | Pr1-deGFP-MGapt | OR2-OR1-Pr1 promoter expressing deGFP-MG aptamer fusion |
| 67736 | Pr2-deGFP-MGapt | OR2-OR1-Pr2 promoter expressing deGFP-MG aptamer fusion |
| 67738 | Pr1-T7RNAP | OR2-OR1-Pr1 promoter expressing T7 RNAP |
| 67739 | Pr2-T7RNAP | OR2-OR1-P2 promoter expressing T7 RNAP |
| 67740 | Pr-deCFP-MGapt | OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion |
| 67741 | PT7-deGFP-MGapt | T7 promoter expressing deGFP-MG aptamer fusion |
| 67743 | Pr-deGFP | Pr promoter expressing deGFP |
| 67744 | Pr-delRBS-deGFP | Pr promoter expressing a deGFP that is missing a ribosomal binding site |