Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase.
Lucas JE, Siegel JB
Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015 Apr 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67721 | pfMDH_WT | Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67724 | pfMDH_N191A | Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67725 | pfMDH_D230A | Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67726 | pfMDH_K295A | Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67728 | pfMDH_N300D | Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67729 | pfMDH_N300S | Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67730 | pfMDH_H303A | Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67731 | pfMDH_R373A | Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67732 | pfMDH_K381A | Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |
| 67733 | pfMDH_Triple | Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli |