Quantitative functional characterization of conserved molecular interactions in the active site of mannitol 2-dehydrogenase.

Lucas JE, Siegel JB
Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015 Apr 2. PubMed Article

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ID	Plasmid	Purpose
67733	pfMDH_Triple	Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67732	pfMDH_K381A	Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67731	pfMDH_R373A	Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67730	pfMDH_H303A	Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67729	pfMDH_N300S	Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67728	pfMDH_N300D	Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67726	pfMDH_K295A	Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67725	pfMDH_D230A	Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67724	pfMDH_N191A	Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
67721	pfMDH_WT	Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli