Tool box: Plasmids for the expression or knockdown of human ARF Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria with N-myristoyltransferases.

Kerr SC, Kahn RA
Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
67306	pDONR221-ARF1	hArf1 with stop codon in Gateway Entry vector
67307	pDONR221-ARF1-no stop	hArf1 without stop codon in Gateway Entry vector
67308	pDONR221-ARF3	hArf3 with stop codon in Gateway Entry vector
67309	pDONR221-ARF3-no stop	hArf3 without stop codon in Gateway Entry vector
67310	pDONR221-ARF4	hArf4 with stop codon in Gateway Entry vector
67311	pDONR221-ARF4-no stop	hArf4 without stop codon in Gateway Entry vector
67312	pDONR221-ARF5	hArf5 with stop codon in Gateway Entry vector
67314	pDONR221-ARF6	hArf6 with stop codon in Gateway Entry vector
67315	pDONR221-ARF6-no stop	hArf6 without stop codon in Gateway Entry vector
67316	pDONR221-ARL1	hArl1 with stop codon in Gateway Entry vector
67317	pDONR221-ARL1-no stop	hArl1 without stop codon in Gateway Entry vector
67318	pDONR221-ARL2	hArl2 with stop codon in Gateway Entry vector
67319	pDONR221-ARL2-no stop	hArl2 without stop codon in Gateway Entry vector
67320	pDONR221-ARL3	hArl3 with stop codon in Gateway Entry vector
67321	pDONR221-ARL3-no stop	hArl3 without stop codon in Gateway Entry vector
67322	pDONR221-ARL4	hArl4 with stop codon in Gateway Entry vector
67323	pDONR221-ARL4-no stop	hArl4 without stop codon in Gateway Entry vector
67324	pDONR221-ARL5A	hArl5a with stop codon in Gateway Entry vector
67325	pDONR221-ARL5A-no stop	hArl5a without stop codon in Gateway Entry vector
67326	pDONR221-ARL5B	hArl5b with stop codon in Gateway Entry vector
67327	pDONR221-ARL5B-no stop	hArl5b without stop codon in Gateway Entry vector
67328	pDONR221-ARL6	hArl6 with stop codon in Gateway Entry vector
67329	pDONR221-ARL6-no stop	hArl6 without stop codon in Gateway Entry vector
67330	pDONR221-ARL4C	hArl4c with stop codon in Gateway Entry vector
67331	pDONR221-ARL4C-no stop	hArl4c without stop codon in Gateway Entry vector
67332	pDONR221-ARL8A	hArl8a with stop codon in Gateway Entry vector
67333	pDONR221-ARL8A-no stop	hArl8a without stop codon in Gateway Entry vector
67334	pDONR221-ARL8B	hArl8b with stop codon in Gateway Entry vector
67335	pDONR221-ARL8B-no stop	hArl8b without stop codon in Gateway Entry vector
67336	pDONR221-ARL4D	hArl4D with stop codon in Gateway Entry vector
67337	pDONR221-ARL4D-no stop	hArl4D without stop codon in Gateway Entry vector
67338	pDONR221-ARL14	hArl14 with stop codon in Gateway Entry vector
67339	pDONR221-ARL14-no stop	hArl14 without stop codon in Gateway Entry vector
67340	pDONR221-ARL11	hArl11 with stop codon in Gateway Entry vector
67341	pDONR221-ARL11-no stop	hArl11 without stop codon in Gateway Entry vector
67342	pDONR221-ARFRP1	hArfrp1 with stop codon in Gateway Entry vector
67343	pDONR221-ARFRP1-no stop	hArfrp1 without stop codon in Gateway Entry vector
67344	pDONR221-SAR1	hSar1 with stop codon in Gateway Entry vector
67345	pDONR221-SAR1-no stop	hSar1 without stop codon in Gateway Entry vector
67346	pDONR221-SARA	hSara with stop codon in Gateway Entry vector
67347	pDONR221-SARA-no stop	hSara without stop codon in Gateway Entry vector
67348	pDSHA-ARF1-Myc	Mammalian expression of hArf1 with C-term Myc tag
67349	pDSHA-ARF3-Myc	Mammalian expression of hArf3 with C-term Myc tag
67350	pDSHA-ARF4-Myc	Mammalian expression of hArf4 with C-term Myc tag
67351	pDSHA-ARF5-Myc	Mammalian expression of hArf5 with C-term Myc tag
67352	pDSHA-ARF6-Myc	Mammalian expression of hArf6 with C-term Myc tag
67353	pDSHA-ARL1-Myc	Mammalian expression of hArl1 with C-term Myc tag
67354	pDSHA-ARL2-Myc	Mammalian expression of hArl2 with C-term Myc tag
67355	pDSHA-ARL3-Myc	Mammalian expression of hArl3 with C-term Myc tag
67356	pDSHA-ARL4-Myc	Mammalian expression of hArl4 with C-term Myc tag
67357	pDSHA-ARL5A-Myc	Mammalian expression of hArl5 with C-term Myc tag
67358	pDSHA-ARL5B-Myc	Mammalian expression of hArl5b with C-term Myc tag
67359	pDSHA-ARL6-Myc	Mammalian expression of hArl6 with C-term Myc tag
67360	pDSHA-ARL4C-Myc	Mammalian expression of hArl4c with C-term Myc tag
67361	pDSHA-ARL8A-Myc	Mammalian expression of hArl8 with C-term Myc tag
67362	pDSHA-ARL8B-Myc	Mammalian expression of hArl8b with C-term Myc tag
67363	pDSHA-ARL4D-Myc	Mammalian expression of hArl4D with C-term Myc tag
67364	pDSHA-ARL14-Myc	Mammalian expression of hArl14 with C-term Myc tag
67365	pDSHA-ARL11-Myc	Mammalian expression of hArl11 with C-term Myc tag
67366	pDSHA-ARFRP1-Myc	Mammalian expression of hArfrp with C-term Myc tag
67367	pDSHA-SAR1-Myc	Mammalian expression of hSar1 with C-term Myc tag
67368	pDSHA-SARA-Myc	Mammalian expression of hSara with C-term Myc tag
67369	pDEST14-ARF1	Expression of native hArf1 in E. coli
67370	pDEST14-ARF3	Expression of native hArf3 in E. coli
67371	pDEST14-ARF4	Expression of native hArf4 in E. coli
67372	pDEST14-ARF5	Expression of native hArf5 in E. coli
67373	pDEST14-ARF6	Expression of native hArf6 in E. coli
67374	pDEST14-ARL1	Expression of native hArl1 in E. coli
67375	pDEST14-ARL2	Expression of native hArl2 in E. coli
67376	pDEST14-ARL3	Expression of native hArl3 in E. coli
67377	pDEST14-ARL4	Expression of native hArl4 in E. coli
67378	pDEST14-ARL5A	Expression of native hArl5 in E. coli
67379	pDEST14-ARL5B	Expression of native hArl5b in E. coli
67380	pDEST14-ARL6	Expression of native hArl6 in E. coli
67381	pDEST14-ARL4C	Expression of native hArl4c in E. coli
67382	pDEST14-ARL8A	Expression of native hArl8 in E. coli
67383	pDEST14-ARL8B	Expression of native hArl8b in E. coli
67384	pDEST14-ARL4D	Expression of native hArl4D in E. coli
67385	pDEST14-ARL14	Expression of native hArl14 in E. coli
67386	pDEST14-ARL11	Expression of native hArl11 in E. coli
67387	pDEST14-ARFRP1	Expression of native hArfrp in E. coli
67388	pDEST14-SAR1	Expression of native hSar1 in E. coli
67389	pDEST14-SARA	Expression of native hSara in E. coli
67390	pDEST47-ARF1-GFP	Mammalian expression of hArf1 with C-term GFP tag
67391	pDEST47-ARF3-GFP	Mammalian expression of hArf3 with C-term GFP tag
67392	pDEST47-ARF4-GFP	Mammalian expression of hArf4 with C-term GFP tag
67393	pDEST47-ARF5-GFP	Mammalian expression of hArf5 with C-term GFP tag
67394	pDEST47-ARF6-GFP	Mammalian expression of hArf6 with C-term GFP tag
67395	pDEST47-ARL1-GFP	Mammalian expression of hArl1 with C-term GFP tag
67396	pDEST47-ARL2-GFP	Mammalian expression of hArl2 with C-term GFP tag
67397	pDEST47-ARL3-GFP	Mammalian expression of hArl3 with C-term GFP tag
67398	pDEST47-ARL4-GFP	Mammalian expression of hArl4 with C-term GFP tag
67399	pDEST47-ARL5A-GFP	Mammalian expression of hArl5 with C-term GFP tag
67400	pDEST47-ARL5B-GFP	Mammalian expression of hArl5b with C-term GFP tag
67401	pDEST47-ARL6-GFP	Mammalian expression of hArl6 with C-term GFP tag
67402	pDEST47-ARL4C-GFP	Mammalian expression of hArl4c with C-term GFP tag
67403	pDEST47-ARL8A-GFP	Mammalian expression of hArl8 with C-term GFP tag
67404	pDEST47-ARL8B-GFP	Mammalian expression of hArl8b with C-term GFP tag
67405	pDEST47-ARL4D-GFP	Mammalian expression of hArl4D with C-term GFP tag
67406	pDEST47-ARL14-GFP	Mammalian expression of hArl14 with C-term GFP tag
67407	pDEST47-ARL11-GFP	Mammalian expression of hArl11 with C-term GFP tag
67408	pDEST47-ARFRP1-GFP	Mammalian expression of hArfrp with C-term GFP tag
67409	pDEST47-SAR1-GFP	Mammalian expression of hSar1 with C-term GFP tag
67410	pDEST47-SARA-GFP	Mammalian expression of hSara with C-term GFP tag
67411	pET-DEST42-ARF1-V5-His6	Expression of hArf1 with C-term V5 and His6 tag in E. coli
67412	pET-DEST42-ARF3-V5-His6	Expression of hArf3 with C-term V5 and His6 tag in E. coli
67413	pET-DEST42-ARF4-V5-His6	Expression of hArf4 with C-term V5 and His6 tag in E. coli
67414	pET-DEST42-ARF5-V5-His6	Expression of hArf5 with C-term V5 and His6 tag in E. coli
67415	pET-DEST42-ARF6-V5-His6	Expression of hArf6 with C-term V5 and His6 tag in E. coli
67416	pET-DEST42-ARL1-V5-His6	Expression of hArl1 with C-term V5 and His6 tag in E. coli
67417	pET-DEST42-ARL2-V5-His6	Expression of hArl2 with C-term V5 and His6 tag in E. coli
67418	pET-DEST42-ARL3	Expression of hArl3 in E. coli
67419	pET-DEST42-ARL4-V5-His6	Expression of hArl4 with C-term V5 and His6 tag in E. coli
67420	pET-DEST42-ARL5A-V5-His6	Expression of hArl5 with C-term V5 and His6 tag in E. coli
67422	pET-DEST42-ARL6-V5-His6	Expression of hArl6 with C-term V5 and His6 tag in E. coli
67423	pET-DEST42-ARL4C-V5-His6	Expression of hArl4c with C-term V5 and His6 tag in E. coli
67424	pET-DEST42-ARL8A-V5-His6	Expression of hArl8 with C-term V5 and His6 tag in E. coli
67425	pET-DEST42-ARL8B-V5-His6	Expression of hArl8b with C-term V5 and His6 tag in E. coli
67426	pET-DEST42-ARL4D-V5-His6	Expression of hArl4D with C-term V5 and His6 tag in E. coli
67427	pET-DEST42-ARL14-V5-His6	Expression of hArl14 with C-term V5 and His6 tag in E. coli
67428	pET-DEST42-ARL11-V5-His6	Expression of hArl11 with C-term V5 and His6 tag in E. coli
67429	pET-DEST42-ARFRP1-V5-His6	Expression of hArfrp with C-term V5 and His6 tag in E. coli
67430	pET-DEST42-SAR1-V5-His6	Expression of hSar1 with C-term V5 and His6 tag in E. coli
67431	pET-DEST42-SARA-V5-His6	Expression of hSara with C-term V5 and His6 tag in E. coli
67432	pDEST40-ARF1-V5-His6	Mammalian expression of hArf1 with C-term V5 and His6 tag
67433	pDEST40-ARF3-V5-His6	Mammalian expression of hArf3 with C-term V5 and His6 tag
67434	pDEST40-ARF4-V5-His6	Mammalian expression of hArf4 with C-term V5 and His6 tag
67436	pDEST40-ARF6-V5-His6	Mammalian expression of hArf6 with C-term V5 and His6 tag
67437	pDEST40-ARL1-V5-His6	Mammalian expression of hArl1 with C-term V5 and His6 tag
67438	pDEST40-ARL2-V5-His6	Mammalian expression of hArl2 with C-term V5 and His6 tag
67440	pDEST40-ARL4-V5-His6	Mammalian expression of hArl4 with C-term V5 and His6 tag
67441	pDEST40-ARL5A-V5-His6	Mammalian expression of hArl5 with C-term V5 and His6 tag
67442	pDEST40-ARL5B-V5-His6	Mammalian expression of hArl5b with C-term V5 and His6 tag
67443	pDEST40-ARL6-V5-His6	Mammalian expression of hArl6 with C-term V5 and His6 tag
67444	pDEST40-ARL4C-V5-His6	Mammalian expression of hArl4c with C-term V5 and His6 tag
67445	pDEST40-ARL8A-V5-His6	Mammalian expression of hArl8 with C-term V5 and His6 tag
67446	pDEST40-ARL8B-V5-His6	Mammalian expression of hArl8b with C-term V5 and His6 tag
67447	pDEST40-ARL4D-V5-His6	Mammalian expression of hArl4D with C-term V5 and His6 tag
67448	pDEST40-ARL14-V5-His6	Mammalian expression of hArl14 with C-term V5 and His6 tag
67449	pDEST40-ARL11-V5-His6	Mammalian expression of hArl11 with C-term V5 and His6 tag
67450	pDEST40-ARFRP1-V5-His6	Mammalian expression of hArfrp with C-term V5 and His6 tag
67451	pDEST40-SAR1-V5-His6	Mammalian expression of hSar1 with C-term V5 and His6 tag
67452	pDEST40-SARA-V5-His6	Mammalian expression of hSara with C-term V5 and His6 tag
67453	pDEST47-ARF1	Mammalian expression of hArf1 with stop codon (native expression)
67454	pDEST47-ARF3	Mammalian expression of hArf3 with stop codon (native expression)
67455	pDEST47-ARF4	Mammalian expression of hArf4 with stop codon (native expression)
67456	pDEST47-ARF5	Mammalian expression of hArf5 with stop codon (native expression)
67457	pDEST47-ARF6	Mammalian expression of hArf6 with stop codon (native expression)
67458	pDEST47-ARL1	Mammalian expression of hArl1 with stop codon (native expression)
67459	pDEST47-ARL2	Mammalian expression of hArl2 with stop codon (native expression)
67460	pDEST47-ARL3	Mammalian expression of hArl3 with stop codon (native expression)
67461	pDEST47-ARL4	Mammalian expression of hArl4 with stop codon (native expression)
67462	pDEST47-ARL5A	Mammalian expression of hArl5 with stop codon (native expression)
67463	pDEST47-ARL5B	Mammalian expression of hArl5b with stop codon (native expression)
67464	pDEST47-ARL6	Mammalian expression of hArl6 with stop codon (native expression)
67465	pDEST47-ARL4C	Mammalian expression of hArl4c with stop codon (native expression)
67466	pDEST47-ARL8A	Mammalian expression of hArl8 with stop codon (native expression)
67467	pDEST47-ARL8B	Mammalian expression of hArl8b with stop codon (native expression)
67468	pDEST47-ARL4D	Mammalian expression of hArl4D with stop codon (native expression)
67469	pDEST47-ARL14	Mammalian expression of hArl14 with stop codon (native expression)
67470	pDEST47-ARL11	Mammalian expression of hArl11 with stop codon (native expression)
67471	pDEST47-ARFRP1	Mammalian expression of hArfrp with stop codon (native expression)
67472	pDEST47-SAR1	Mammalian expression of hSar1 with stop codon (native expression)
67473	pDEST47-SARA	Mammalian expression of hSara with stop codon (native expression)
67474	pDEST17-6xHis-ARL2	Expression of hArl2 with N-term His6 tag in E. coli
67479	pCMV0-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67480	pCMV1-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67481	pCMV2-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67482	pCMV3-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67483	pCMV4-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67484	pCMV5-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67485	pCMV6-OCT-HA-eGFP	Targets HA-eGFP to mitochondrial matrix
67486	pCMV0-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67487	pCMV1-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67488	pCMV2-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67489	pCMV3-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67490	pCMV4-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67491	pCMV5-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)
67492	pCMV6-SMAC-HA-eGFP	Targets HA-eGFP to mitochondrial intermembrane space (IMS)