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Coexpression of proteins with methionine aminopeptidase and/or N-myristoyltransferase in Escherichia coli to increase acylation and homogeneity of protein preparations.

Van Valkenburgh HA, Kahn RA
Methods Enzymol. 2002;344:186-93. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
67475pMON-HsNMT1Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria
67476pMON-HsNMT2Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria
117476pHV738Expression of NMT1
117477pHV739Expression of NMT2

Antibodies from Article