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Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.

Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera D, Gonda TJ, Alexandrov K
J Biotechnol. 2015 Feb 10;195:1-7. doi: 10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67135pCellFree_G01Gateway destination vector for cell-free expression with N-term 8xHis tag
67136pCellFree_G02Gateway destination vector for cell-free expression with C-term 8xHis tag
67137pCellFree_G03Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag
67138pCellFree_G04Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag
67139pCellFree_G05Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag
67140pCellFree_G06Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag
67141pCellFree_G07Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag
67142pCellFree_G08Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag
67143pCellFree_G09Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag
67144pCellFree_G10Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Antibodies from Article