On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant.
Coffa G, Imber AN, Maguire BC, Laxmikanthan G, Schneider C, Gaffney BJ, Brash AR
J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66846 | pET30Xa-SBL1(160E) | Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag |
| 66872 | pET30Xa-SBL1(160S) | Expresses WTsoybean lipoxygenase-1 160S with N-term Histag |
| 66875 | pET30Xa-SBL1(160E,NoCys) | Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag |
| 66877 | pET30Xa-SBL1(160S,NoCys) | Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag |
| 66879 | pET30-SBL1(160E,HisTagDel) | Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed |
| 66880 | pET30-SBL1(160S,HisTagDel) | Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed |