RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.
Filonov GS, Jaffrey SR
Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 10.1002/9780470559277.ch150174.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66843 | pET28c-F30-2xdBroccoli | Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells |
| 66845 | pAV5S-F30-2xdBroccoli | Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells |