Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Floe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66818 | pCCL-PGK-SPdCas9-BFP-DNMT1 | dCas9 fused to BFP and the human DNMT1 catalytic domain |
| 66819 | pCCL-PGK-SPdCas9-BFP-DNMT3A | dCas9 fused to BFP and the human DNMT3A catalytic domain |
| 66820 | pCCL-PGK-SPdCas9-BFP-DNMT3B | dCas9 fused to BFP and the human DNMT3B catalytic domain |
| 66821 | pCCL-PGK-SPdCas9-BFP-EGFP | dCas9 fused to BFP and EGFP |
| 71213 | pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A) | dCas9 fused to BFP and the human DNMT3A catalytically inactive domain |
| 71214 | pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A) | dCas9 fused to BFP and the human DNMT3B catalytically inactive domain |
| 71215 | pLenti-EF1a-SPdCas9-EGFP-2A-Blast | dCas9 fused to EGFP |
| 71216 | pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast | dCas9 fused to human DNMT3A catalytic domain |
| 71217 | pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast | dCas9 fused to human DNMT3B catalytic domain |
| 71218 | pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast | dCas9 fused to the human DNMT3A catalytically inactive domain |
| 71219 | pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast | dCas9 fused to the human DNMT3B catalytically inactive domain |