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Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells.

Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes NI, Feist AM, Vander Heiden MG, Metallo CM
Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
66802pSLIK-IDH1-FLAGTet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector
66803pSLIK-IDH1-R132H-FLAGTet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector
66806pSLIK-IDH2-FLAGTet-inducible expression of IDH2 in mammalian cells
66807pSLIK-IDH2-R172K-FLAGTet-inducible expression of mutant IDH2 in mammalian cells
66844pSLIK-GFPTet-inducible expression of GFP in mammalian cells

Antibodies from Article