Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.
Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
J Biol Chem. 2014 May 23;289(21):14569-82. doi: 10.1074/jbc.M114.563031. Epub 2014 Mar 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121151 | pRK5-HA-Ubiquitin-K6 | Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines |
| 121152 | pRK5-HA-Ubiquitin-K11 | Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines |
| 121153 | pRK5-HA-Ubiquitin-K6R | Mammalian expression of HA tagged ubiquitin K6R |
| 121154 | pRK5-HA-Ubiquitin-K11R | Mammalian expression of HA tagged ubiquitin K11R |
| 121155 | pRK5-HA-Ubiquitin-K27R | Mammalian expression of HA tagged ubiquitin K27R |
| 121156 | pRK5-HA-Ubiquitin-K27R-K29R | Mammalian expression of HA tagged ubiquitin K27R and K29R |
| 127588 | GFP-Miro1 S156A | Phospho-mutant of S156 |
| 127589 | GFP-Miro1 S156E | Phospho-mimetic of S156 |
| 127621 | GFP-Trak1 | N-terminal GFP-tagged mouse Trak1 |
| 127622 | GFP-Trak2 | N-terminal GFP-tagged mouse Trak2 |