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Controlled expression of transgenes introduced by in vivo electroporation.

Matsuda T, Cepko CL
Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104 (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
13764pNrl-DsRed
13765pCrx-DsRed
13766pNdrg4-DsRed
13767pHes1-DsRed
13769pCALNL-DsRedCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
13770pCALNL-GFPCre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
13771pCAFNF-DsRedFlp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.
13772pCAFNF-GFPFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.
13775pCAG-CreMammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer
13776pCAG-Cre:GFPMammalian expression of Cre recombinase fused to GFP
13777pCAG-ERT2CreERT2Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)
13779pRho-Cre
13780pNrl-Cre
13786pCALSL-mir30
13787pCAG-FlpeMammalian expression of enhanced form of Flp recombinase
13788pCAG-Flpe:GFPMammalian expression of enhanced form of Flp recombinase fused to GFP
14756pCAG-FlpeERT2
14757pCAG-mGFP
14758pCAG-mir30
14760pCAG-GFPd2
14796pClusterin-ERT2CreERT2
14797pCAG-CreERT2Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)
14808pHes1-GFPd2

Antibodies from Article