Charles Gersbach Lab: Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic Cellular Differentiation in Primary Human Cells
Gersbach C
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66631 | pLV hMyoD-IRES-dsRed | Co-expresses human MyoD and dsRed |
| 66632 | pLV floxed hMyoD-IRES-dsRed | co-expresses CRE excisable human MyoD and dsRed |
| 66707 | pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP | expresses a single gRNA and VP64-dCas9-VP64 |
| 66708 | pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites) | expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed |