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YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.

Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
Nucleic Acids Res. 2015 May 8. pii: gkv464. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65314HcKan_Preceiving vector of promoters for the construction of standard promoter parts in YeastFab work
65315HcKan_Oreceiving vector of ORFs for the construction of standard ORF parts in YeastFab work
65316HcKan_Treceiving vector of terminators for the construction of standard terminator parts in YeastFab work
65318POT2-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65319POT3-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65320POT4-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65321POT5-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65322POT6-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65323POT7-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65324POT8-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65325POT9-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65326POT10-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65327POT11-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
65328pYG026reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.
65329pRS414-URRCEN plasmid as receiving vector for exogenous pathway
65330pRS424-URRhigh copy number plasmid as receiving vector for exogenous pathway
65331pMV-URR1standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs
65332pMV-URR2standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs
65333pMV-HIS3standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs
65334pMV-LEU2standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs
65335pMV-TRP1standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs
65336HCKan-P-TDH3construction of TDH3 promoter in HCKan-P receiving vector
65337HCKan-P-TEF2construction of TEF2 promoter in HCKan-P receiving vector
65338HCKan-P-CYC1construction of CYC1 promoter in HCKan-P receiving vector
65339HCKan-T-ADH1construction of ADH1 terminator in HCKan-P receiving vector
65340HCKan-T-TEF2construction of TEF2 terminator in HCKan-P receiving vector
65341PMV-ycrtEsysthesis of crtE gene in the beta-carotene pathway
65342PMV-ycrtIsysthesis of crtI gene in the beta-carotene pathway
65343PMV-ycrtYBsysthesis of crtYB gene in the beta-carotene pathway
65344HCKan-O-ycrtEconstruction of ycrtE gene in HCKan-O receiving vector
65345HCKan-O-ycrtIconstruction of ycrtI gene in HCKan-O receiving vector
65346HCKan-O-ycrtYBconstruction of ycrtYB gene in HCKan-O receiving vector
65347pKMV-VioAsysthesis of vioA gene in the violacein pathway
65348pKMV-VioBsysthesis of vioB gene in the violacein pathway
65349pKMV-VioCsysthesis of vioC gene in the violacein pathway
65350pKMV-VioDsysthesis of vioD gene in the violacein pathway
65351pKMV-VioEsysthesis of vioE gene in the violacein pathway
65352POT2-TDH3p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
65353POT2-TEF2p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
65354POT2-CYC1p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
65355POT4-TDH3p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
65356POT4-TEF2p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
65357POT4-CYC1p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
65358POT5-TDH3p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway
65359POT5-TEF2p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway
65360POT5-CYC1p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway

Antibodies from Article